9Y0T

Crystal structure of human MAIT A-F7 TCR-MR1*02-5-OP-RU complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	218.226, 71.054, 144.176
Unit cell angles	90.00, 104.57, 90.00

Refinement procedure

Resolution	46.620 - 2.300
R-factor	0.1923
Rwork	0.191
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	94639	4603
<I/σ(I)>	10
Completeness [%]	99.4
Redundancy	3.9
CC(1/2)	0.997	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, BTP, Cacl2