9Y0G
Crystal structure of NRas-G12D in complex with GDP and compound 1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.588, 45.937, 88.967 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.21470 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.840 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.125 | 0.837 |
| Rmeas | 0.136 | 1.018 |
| Rpim | 0.053 | 0.562 |
| Total number of observations | 219234 | 1919 |
| Number of reflections | 34606 | 626 |
| <I/σ(I)> | 12.4 | 1.2 |
| Completeness [%] | 88.5 | |
| Redundancy | 6.3 | 3.1 |
| CC(1/2) | 0.996 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M Sodium Citrate pH5.5, 0.1M MgCl2, 16-22% Peg 4000 |
