9XYW

Crystal structure of the maize chloroplastic non-photosynthetic NADP(+)-dependent malic enzyme

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 2 21
Unit cell lengths	92.453, 108.115, 168.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.232 - 2.550
R-factor	0.2467
Rwork	0.243
R-free	0.30830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.753
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.232	2.620
High resolution limit [Å]	2.550	2.550
Rmerge	0.112	1.832
Rmeas	0.122	2.003
Rpim	0.049	0.803
Number of reflections	55927	4519
<I/σ(I)>	9.7	1
Completeness [%]	99.9	100
Redundancy	6	6.2
CC(1/2)	0.998	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	8% w/v PEG20000, 8% w/v PEG550 MME, 50 mM Tris-HCl, pH 8.5, 1.2 M sodium formate