9XYT
Structure of an ancestral glucokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.295, 92.831, 123.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.420 - 2.000 |
| R-factor | 0.2297 |
| Rwork | 0.229 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.282 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.195 | |
| Rpim | 0.078 | 2.871 |
| Number of reflections | 66106 | 2903 |
| <I/σ(I)> | 6.8 | 0.1 |
| Completeness [%] | 99.1 | 88.3 |
| Redundancy | 6 | |
| CC(1/2) | 0.985 | 0.052 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Tris-HCl pH=7.5, 25 % w/v PEG 2000 MME, 0.3 M sodium acetate |
