9XYS
Crystal structure of juvenile hormone acid methyltransferase CfJHAMT in complex with SAH and juvenile hormone III acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.923, 40.744, 96.761 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 37.580 - 1.770 |
| R-factor | 0.1847 |
| Rwork | 0.182 |
| R-free | 0.23252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.855 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.740 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.040 | 0.531 |
| Rmeas | 0.049 | 0.648 |
| Rpim | 0.027 | 0.365 |
| Total number of observations | 77103 | 10688 |
| Number of reflections | 25705 | 3745 |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 96.0 | |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.999 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 296 | 0.1 M magnesium formate and 15% (w/v) PEG 3350 |
