9XYO
Crystal structure of juvenile hormone acid methyltransferase JHAMT from Choristoneura fumiferana (CfJHAMT) in complex with SAH (crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.6039 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.925, 39.224, 104.674 |
| Unit cell angles | 90.00, 97.95, 90.00 |
Refinement procedure
| Resolution | 36.710 - 2.100 |
| R-factor | 0.21036 |
| Rwork | 0.208 |
| R-free | 0.25353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.852 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 | 0.768 |
| Rmeas | 0.067 | 0.912 |
| Rpim | 0.026 | 0.351 |
| Total number of observations | 104888 | 14315 |
| Number of reflections | 15890 | 2224 |
| <I/σ(I)> | 15.3 | 1.9 |
| Completeness [%] | 95.8 | |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.999 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 296 | 0.1 M Magnesium formate, 15 %(w/v) PEG 3350 |
