9XVQ

Crystal structure of the NFIA-N0 DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97914
Spacegroup name	I 21 21 21
Unit cell lengths	48.501, 113.243, 128.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.210 - 1.760
R-factor	0.1962
Rwork	0.195
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.210	1.810
High resolution limit [Å]	1.760	1.760
Number of reflections	35439	2582
<I/σ(I)>	20.7
Completeness [%]	99.9
Redundancy	11.7
CC(1/2)	1.000	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M sodium phosphate dibasic dihydrate and 20% (w/v) PEG 3350