9XVO

Crystal structure of NFIC monomer bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	61.917, 66.273, 140.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.140 - 2.500
R-factor	0.2059
Rwork	0.204
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.272
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.230	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	20711	2304
<I/σ(I)>	19.6	2.3
Completeness [%]	100.0
Redundancy	8.7
CC(1/2)	0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES monohydrate (pH 6.0) and 14% (w/v) PEG 4000