9XVF
Crystal structure of NFIA monomer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-06 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 83.677, 48.463, 105.882 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.600 |
| R-factor | 0.19591 |
| Rwork | 0.193 |
| R-free | 0.24759 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.945 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.460 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 25334 | 2282 |
| <I/σ(I)> | 7.6 | 1.2 |
| Completeness [%] | 95.3 | 70.8 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.987 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5% (v/v) (+/-)-2-methyl-2,4-pentanediol, 0.1 M HEPES (pH 7.5), and 10% (w/v) PEG 10000 |
