9XVD

Crystal structure of NFIC homodimer bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	83.457, 84.290, 146.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.270 - 2.600
R-factor	0.1945
Rwork	0.191
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.077
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	32646	3210
<I/σ(I)>	23.3
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.997	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES monohydrate (pH 6.0), 22% (v/v) polyethylene glycol (PEG) 400