9XV8
Catalytic domain of N1484 E121S variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.463, 43.941, 84.151 |
| Unit cell angles | 89.97, 90.02, 87.80 |
Refinement procedure
| Resolution | 28.050 - 1.640 |
| R-factor | 0.17394 |
| Rwork | 0.171 |
| R-free | 0.22517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.404 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.050 | 1.699 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.143 | 0.523 |
| Number of reflections | 57576 | 5552 |
| <I/σ(I)> | 10.9 | 3.1 |
| Completeness [%] | 95.2 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.989 | 0.711 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.26M ammonium sulfate, 0.1M HEPES (pH 7.5) |
