9XV5

Catalytic domain of N1484

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	34.239, 84.212, 43.853
Unit cell angles	90.00, 92.78, 90.00

Refinement procedure

Resolution	43.800 - 1.400
R-factor	0.15398
Rwork	0.153
R-free	0.17684
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.830
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.800	1.448
High resolution limit [Å]	1.400	1.400
Rmerge	0.077	0.580
Number of reflections	48448	4862
<I/σ(I)>	12.2	2.2
Completeness [%]	98.7
Redundancy	3.7
CC(1/2)	0.990	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% (v/v) PEG 8000, 0.1M imidazole (pH 8.0), 0.2M sodium chloride