9XTH

The complex structure of antibody CAV-CH76 bound to the hemagglutinin of influenza B virus (HA_B/Guangdong-Yuexiu/120/2022)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	50.207, 141.509, 53.079
Unit cell angles	90.00, 105.54, 90.00

Refinement procedure

Resolution	30.970 - 3.010
R-factor	0.1995
Rwork	0.197
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.345
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.970	3.118
High resolution limit [Å]	3.010	3.010
Number of reflections	13861	1373
<I/σ(I)>	50.78
Completeness [%]	97.6
Redundancy	6.2
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350