9XSX

the complex structure of antibody CF22 bound to the hemagglutinin of influenza B virus (HA_B/Guangdong-Yuexiu/120/2022).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	103.540, 103.540, 164.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.420 - 2.980
R-factor	0.2116
Rwork	0.210
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.456
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.510	3.090
High resolution limit [Å]	2.980	2.980
Number of reflections	17430	11705
<I/σ(I)>	13.8
Completeness [%]	99.1
Redundancy	1.2
CC(1/2)	1.000	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350