9XS1
Crystal structure of FOXM1 DNA binding domain to specific dsDNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 72.210, 45.610, 213.494 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 30.400 - 2.500 |
| R-factor | 0.2144 |
| Rwork | 0.213 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.086 | 0.891 |
| Number of reflections | 24027 | 2686 |
| <I/σ(I)> | 10.8 | 2.6 |
| Completeness [%] | 98.4 | 98.8 |
| Redundancy | 6.6 | 7.1 |
| CC(1/2) | 0.997 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 10% PEG 20000, 0.1 M Sodium citrate tribasic dihydrate (pH 5.0) and 0.2 M Magnesium chloride hexahydrate |
