9XRB

Maltose-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-11
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	70.788, 65.047, 82.651
Unit cell angles	90.00, 96.84, 90.00

Refinement procedure

Resolution	42.800 - 2.100
R-factor	0.201
Rwork	0.198
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.008
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	2.160
High resolution limit [Å]	2.100	2.100
Rmeas	0.047	0.963
Rpim	0.021	0.451
Number of reflections	43532	3489
<I/σ(I)>	6.6	1.6
Completeness [%]	99.6
Redundancy	6.6
CC(1/2)	1.000	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	1.4 M sodium citrate