9XRA

Maltose-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-11
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	70.093, 65.143, 82.702
Unit cell angles	90.00, 96.64, 90.00

Refinement procedure

Resolution	39.820 - 2.100
R-factor	0.1933
Rwork	0.190
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.898
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.570	2.160
High resolution limit [Å]	2.100	2.100
Rmeas	0.047	0.859
Rpim	0.018	0.322
Number of reflections	43392	3469
<I/σ(I)>	24.9	2.5
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	1.000	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	1.4 M sodium citrate