9XPR

Maltose-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-19
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	70.443, 64.765, 82.507
Unit cell angles	90.00, 97.25, 90.00

Refinement procedure

Resolution	33.520 - 1.600
R-factor	0.1939
Rwork	0.192
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.006
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	1.630
High resolution limit [Å]	1.600	1.600
Rmeas	0.027	0.794
Rpim	0.017	0.490
Number of reflections	96832	4579
<I/σ(I)>	19.7	1.8
Completeness [%]	99.7	96.3
Redundancy	4.5	4.6
CC(1/2)	1.000	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	1.4 M sodium citrate