9XOR
Paenibacillus maltose-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-19 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.019, 66.940, 82.766 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 35.040 - 1.440 |
| R-factor | 0.1878 |
| Rwork | 0.186 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.821 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.043 | 1.076 |
| Rpim | 0.027 | 0.661 |
| Number of reflections | 134536 | 6264 |
| <I/σ(I)> | 16.1 | 1.6 |
| Completeness [%] | 99.3 | 93.4 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.999 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 7.5 | 293 | 1.4 M sodium citrate |
