9XNL
Grass carp IFNd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2021-10-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.940, 40.130, 89.680 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 33.230 - 1.910 |
| R-factor | 0.20033 |
| Rwork | 0.198 |
| R-free | 0.24821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-3000 |
| Data scaling software | pointless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.230 | 1.910 |
| High resolution limit [Å] | 1.190 | 1.910 |
| Rmerge | 0.068 | 0.804 |
| Number of reflections | 25691 | 25693 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2M ammonium sulfate, 0.1M BIS-TRIS pH 6.5, 25% w/v PEG 3350 |
