9XMR
the structure of HLA*1101 complex with peptide from H1N1 NP342-351 RVSSFIRGKK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-19 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.844, 67.788, 73.401 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 21.560 - 2.600 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.563 | 2.688 |
| High resolution limit [Å] | 2.595 | 2.595 |
| Rmerge | 0.111 | 0.593 |
| Number of reflections | 14395 | 1267 |
| <I/σ(I)> | 12.76 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M Bis-Tris and 20% w/v polyethylene glycol monomethyl ether 5000 |
