9XLC

Crystal structure of the GH16 carrageenase SfGH16 from Saccharicrinis fermentans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	58.424, 68.300, 84.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.040 - 2.000
R-factor	0.2043
Rwork	0.201
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.888
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.120	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	23567	1710
<I/σ(I)>	9.4
Completeness [%]	99.8	100
Redundancy	6.5	6.8
CC(1/2)	0.892	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1M Bis-Tris pH5.5, 25%PEG3350, 0.2M ammonium acetate