9XL7

Crystal structure of the complex of the GH16 carrageenase SfGH16 from Saccharicrinis fermentans with an oligotetrasaccharide of kappa-carrageenan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	58.342, 67.820, 84.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.990 - 1.700
R-factor	0.1661
Rwork	0.164
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.056
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	1.730
High resolution limit [Å]	1.700	1.700
Rmerge		0.630
Number of reflections	37591	1962
<I/σ(I)>	13.4
Completeness [%]	100.0	100
Redundancy	12.4	12.9
CC(1/2)	0.996	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.2 M Sodium citrate tribasic dihydrate, 20% w/v Polyethylene glycol 3,350