9XKT

Crystal structure of the complex of the GH16 carrageenase SfGH16 from Saccharicrinis fermentans with an oligotetrasaccharide of iota-carrageenan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	57.692, 67.878, 84.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.860 - 1.440
R-factor	0.1489
Rwork	0.148
R-free	0.17530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.064
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.910	1.520
High resolution limit [Å]	1.440	1.440
Rmerge	0.081	0.654
Rmeas	0.092	0.747
Number of reflections	61087	8793
<I/σ(I)>	18.2	3.1
Completeness [%]	100.0
Redundancy	8.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M Sodium citrate tribasic dihydrate pH 5.60, 20% v/v 2-Propanol