9XHO
The crystal structure of SsBcmD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.110, 98.068, 111.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.034 - 1.907 |
| R-factor | 0.181059617993 |
| Rwork | 0.179 |
| R-free | 0.22225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.889 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.907 | 1.910 |
| Rmerge | 0.175 | 1.040 |
| Number of reflections | 74139 | 3637 |
| <I/σ(I)> | 0.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.995 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.5 M Sodium chloride, 0.1 M imidazole hydrochloric acid pH 8.0 |
