9XF9
Crystal structure of Kasokero virus cap- snatching endonuclease in complex with WXS
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.320, 80.600, 116.140 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 28.990 - 1.850 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.21430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.042 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.059 | 0.311 |
| Number of reflections | 57733 | 3097 |
| <I/σ(I)> | 14.9 | 2.9 |
| Completeness [%] | 98.5 | 86.3 |
| Redundancy | 4.1 | 2.7 |
| CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0 |
