9XAW
Structure of the SARS-CoV-2 Spike N-terminal domain(NTD) in complex with the BLN8 Fab
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-15 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.940, 117.493, 138.049 |
| Unit cell angles | 110.91, 90.27, 96.37 |
Refinement procedure
| Resolution | 64.400 - 3.130 |
| R-factor | 0.2341 |
| Rwork | 0.232 |
| R-free | 0.28490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.598 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.400 | 3.210 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Number of reflections | 73865 | 5419 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.909 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.15 M Lithium sulfate monohydrate, 0.1 M Citric acid pH 3.5, 18% w/v Polyethylene glycol 6,000, 0.1 M Sodium iodide |
