9XAS
Crystal Structure of Sensory Appendage Protein (AgSAP2) mutant from Anopheles gambiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-24 |
| Detector | STFC Large Pixel Detector |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.562, 44.319, 54.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.470 - 1.310 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n8v |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.830 | 1.340 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rpim | 0.021 | 0.295 |
| Number of reflections | 19810 | 583 |
| <I/σ(I)> | 55.27 | 2.7 |
| Completeness [%] | 95.4 | |
| Redundancy | 11.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Potassium thiocyanate and 30% w/v PEG 2000 MME |
