9X8Z

LppB in Complex with LppA leader peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	52.858, 55.595, 83.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.850 - 2.100
R-factor	0.2057
Rwork	0.201
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	27534	2023
<I/σ(I)>	11.69
Completeness [%]	98.2
Redundancy	6.78
CC(1/2)	0.998	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% (w/v) PEG 1000, 100mM Potassium phosphate monobasic/Sodium phosphate dibasic, pH 6.2, 200mM Sodium chloride