9X8U
Crystal structure of the human GAS41 YEATS domain in complex with an acetylated YFV capsid peptide (K8ac)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-09 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.226, 78.210, 76.085 |
| Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
| Resolution | 38.783 - 3.001 |
| R-factor | 0.2133 |
| Rwork | 0.207 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.207 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.792 | 3.117 |
| High resolution limit [Å] | 3.001 | 3.001 |
| Number of reflections | 14603 | 1332 |
| <I/σ(I)> | 9.03 | |
| Completeness [%] | 96.5 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.913 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | Sodium sulfate , Bis-Tris propane (pH= 6.5), PEG 3350 |
