9X8S
Crystal structure of the human GAS41 YEATS domain in complex with an acetylated YFV capsid peptide (K4ac)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-09 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.979, 80.575, 72.398 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 40.288 - 1.696 |
| R-factor | 0.1868 |
| Rwork | 0.186 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 1.756 |
| High resolution limit [Å] | 1.690 | 1.696 |
| Rmerge | 0.042 | |
| Number of reflections | 76703 | 10276 |
| <I/σ(I)> | 34.3 | |
| Completeness [%] | 94.2 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.998 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | Sodium sulfate , Bis-Tris propane (pH= 6.5), PEG 3350 |
