9X81

Crystal structure of the C-terminal of an alpha-amylase family glycosyl hydrolase from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	47.700, 67.830, 86.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.000 - 1.420
R-factor	0.17796
Rwork	0.176
R-free	0.21144
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.892
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.500	1.460
High resolution limit [Å]	1.420	1.420
Number of reflections	53585	3925
<I/σ(I)>	16.3	2.3
Completeness [%]	99.9
Redundancy	12.1
CC(1/2)	0.999	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.2 M Magnesium chloride hexahydrate, 20 % w/v PEG 6000, 0.1 M Tris 8.0