9X6W
Crystal structure of NodD-EBD (Effector Binding Domain) from Rhizobium leguminosarum bv. vicae 3841
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-25 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 192.774, 192.774, 153.297 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.860 - 3.300 |
| R-factor | 0.2232 |
| Rwork | 0.222 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.270 |
| Data reduction software | autoPX |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.416 |
| High resolution limit [Å] | 3.298 | 3.298 |
| Rmerge | 0.156 | |
| Number of reflections | 49459 | 49459 |
| <I/σ(I)> | 13.83 | |
| Completeness [%] | 99.1 | |
| Redundancy | 7.75 | |
| CC(1/2) | 0.991 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M HEPES pH 7.0, 0.1 M potassium chloride, 15% w/v polyethylene glycol 5,000 MME |
