9X6C

crystal structure of an ATP bound anti-phage protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-04
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97911
Spacegroup name	I 2 2 2
Unit cell lengths	116.530, 120.430, 121.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.120 - 2.660
R-factor	0.1927
Rwork	0.190
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.120	2.770
High resolution limit [Å]	2.660	2.660
Number of reflections	24880	2701
<I/σ(I)>	11.68
Completeness [%]	99.6
Redundancy	13.5
CC(1/2)	0.998	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, sodium formate, Tris, gamma-PGA, PEG 4000