9X5I

Crystal structure of Medicago truncatula DELLA1-GRAS domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07066
Spacegroup name	P 32 2 1
Unit cell lengths	91.441, 91.441, 229.079
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.180 - 2.000
R-factor	0.1821
Rwork	0.181
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	76178	6396
<I/σ(I)>	38.8
Completeness [%]	100.0
Redundancy	18.1
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289.15	0.1 M HEPES pH 8.1, 0.05 M MgCl2, and 30% (v/v) PEG400