9X5B
Crystal structure of Fgm3 in complex with PLP and L-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.550, 97.779, 69.763 |
| Unit cell angles | 90.00, 116.10, 90.00 |
Refinement procedure
| Resolution | 62.650 - 2.180 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.866 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.650 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.210 | 0.804 |
| Rmeas | 0.252 | 0.982 |
| Rpim | 0.139 | 0.558 |
| Number of reflections | 43839 | 6350 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 | 5.8 |
| CC(1/2) | 0.988 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M Sodium acetate trihydrate, 0.1 M Sodium HEPES, 25% w/v PEG 3350, pH 7.5 |
