9X51
Crystal structure of Fgm3 in complex with PLP and 4(S)-OH-L-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.310, 97.400, 69.790 |
| Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
| Resolution | 59.090 - 1.480 |
| R-factor | 0.1567 |
| Rwork | 0.156 |
| R-free | 0.17420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.100 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.084 | 0.938 |
| Rmeas | 0.092 | 1.091 |
| Rpim | 0.037 | 0.540 |
| Total number of observations | 803731 | 37329 |
| Number of reflections | 135791 | 9508 |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 98.5 | |
| Redundancy | 5.9 | 3.9 |
| CC(1/2) | 0.998 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 7.5% w/v PEG 20000/7.5% v/v PEG 550 MME, 0.1 M Tris, 0.08 M Sodium formate, pH 7.5 |
