9X50

Crystal structure of Fgm3 in complex with PLP and L-Ala

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	69.803, 98.013, 69.827
Unit cell angles	90.00, 116.35, 90.00

Refinement procedure

Resolution	36.810 - 2.100
R-factor	0.1528
Rwork	0.151
R-free	0.18730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.580	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.058	0.153
Rmeas	0.080	0.216
Rpim	0.054	0.152
Number of reflections	47945	3524
<I/σ(I)>	8.8
Completeness [%]	97.5
Redundancy	2.8	1.6
CC(1/2)	0.980	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M MES, 6.25 % w/v PEG 2000, 6.25 % w/v PEG 3350, 6.25 % w/v PEG 4000, 6.25 % w/v PEG 5000 MME, pH 6.5