9X4W
Crystal structure of Fgm3 in complex with PLP and L-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.313, 96.849, 69.713 |
| Unit cell angles | 90.00, 116.60, 90.00 |
Refinement procedure
| Resolution | 61.980 - 1.860 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.980 | 1.970 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.203 | 0.851 |
| Rmeas | 0.220 | 0.936 |
| Rpim | 0.084 | 0.383 |
| Number of reflections | 65436 | 8103 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 95.6 | |
| Redundancy | 6.7 | 5.7 |
| CC(1/2) | 0.991 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M SPG and 25% w/v PEG 1500, pH 9.0 |
