9X4V
Crystal structure of Fgm3 in complex with PLP and L-Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.380, 97.853, 69.690 |
| Unit cell angles | 90.00, 116.27, 90.00 |
Refinement procedure
| Resolution | 59.050 - 1.900 |
| R-factor | 0.1544 |
| Rwork | 0.153 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.490 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.138 | 0.622 |
| Rmeas | 0.151 | 0.704 |
| Rpim | 0.060 | 0.321 |
| Total number of observations | 404345 | 42430 |
| Number of reflections | 65508 | 9473 |
| <I/σ(I)> | 11.8 | 2.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.2 | 4.5 |
| CC(1/2) | 0.995 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M PCTP and 25% w/v PEG 1500, pH 8.0 |
