9X4U
Crystal structure of Fgm3 in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-05 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.804, 97.693, 124.678 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 21.900 - 1.670 |
| R-factor | 0.1793 |
| Rwork | 0.178 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.905 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.420 | 1.710 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.056 | 0.142 |
| Number of reflections | 191763 | 13205 |
| <I/σ(I)> | 12.7 | 5.7 |
| Completeness [%] | 99.3 | 92.5 |
| Redundancy | 5.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.03M Sodium fluoride, 0.03M Sodium bromide, 0.03M Sodium iodide, 0.1 M Sodium HEPES, 0.1 M MOPS (acid), 20% v/v PEG 500 MME, 10% w/v PEG 20000, pH 7.5 |
