9X2X

GSK3beta complexed with BiS-5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	88.710, 91.740, 119.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.510 - 1.790
R-factor	0.1781
Rwork	0.177
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.227
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	1.830
High resolution limit [Å]	1.790	1.790
Number of reflections	177361	12685
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	1.000	0.247

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.15M DL-malic acid, 20% PEG 3350