9X2V

GSK3beta complexed with BiS-3

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.350, 75.310, 85.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.920 - 1.390
R-factor	0.1795
Rwork	0.179
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.035
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	1.420
High resolution limit [Å]	1.390	1.390
Number of reflections	161449	11386
<I/σ(I)>	9.6
Completeness [%]	99.9
Redundancy	4.4
CC(1/2)	0.997	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M sodium malonate pH 7.0, 20% PEG 3350