9X2U

GSK3beta complexed with BiS-2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	89.310, 92.830, 121.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.420 - 2.070
R-factor	0.198
Rwork	0.197
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.546
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.420	2.120
High resolution limit [Å]	2.070	2.070
Number of reflections	118598	8493
<I/σ(I)>	4.9
Completeness [%]	100.0
Redundancy	4.2
CC(1/2)	0.987	0.291

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.2M sodium malonate pH 7.0, 20% PEG 3350