9X2R

Crystal structure of Medicago truncatula NSP1-NSP2 heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL17B
Synchrotron site	NFPSS
Beamline	BL17B
Temperature [K]	80
Detector technology	CCD
Collection date	2021-05-07
Detector	RAYONIX MX-300
Wavelength(s)	0.97853
Spacegroup name	C 1 2 1
Unit cell lengths	191.643, 46.390, 127.117
Unit cell angles	90.00, 122.03, 90.00

Refinement procedure

Resolution	30.890 - 2.410
R-factor	0.1985
Rwork	0.196
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	36559	3468
<I/σ(I)>	13.8
Completeness [%]	98.9
Redundancy	6.6
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	0.1 M Bis-Tris pH 6.5,0.2 M L-proline,10% v/v PEG 3350