9X2Q

GSK3beta complexed with BiS-1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	88.740, 92.170, 119.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 1.680
R-factor	0.1736
Rwork	0.173
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.466
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	1.740
High resolution limit [Å]	1.680	1.680
Number of reflections	112092	7873
<I/σ(I)>	13.7
Completeness [%]	100.0
Redundancy	14.11
CC(1/2)	0.999	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.2M L-proline, 0.1M HEPES-Na pH 7.5, 10% PEG 3350