9X1I
Structure of endo-beta-N-acetylglucosaminidase HS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.927, 135.567, 238.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.050 - 1.810 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.810 |
| Rmerge | 0.085 | 0.686 |
| Rmeas | 0.093 | 0.748 |
| Rpim | 0.036 | 0.292 |
| Number of reflections | 212496 | 10040 |
| <I/σ(I)> | 12.9 | 2.8 |
| Completeness [%] | 99.8 | 96.4 |
| Redundancy | 6.6 | 6.3 |
| CC(1/2) | 0.999 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.0M Ammonium sulfate, 0.1M Bis-Tris-HCl buffer |
