9X15

Crystal structure of Frog M-ferritin E130A_L165D_K168E_H169D mutants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	103
Detector technology	CCD
Collection date	2015-05-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	F 4 3 2
Unit cell lengths	183.762, 183.762, 183.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.120 - 1.450
Rwork	0.123
R-free	0.15100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.017
RMSD bond angle	1.845
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0431 (REFMACAT 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.120	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	47470	1839
<I/σ(I)>	30.2
Completeness [%]	100.0
Redundancy	14
CC(1/2)	1.000	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.0 M MgCl2 and 100 mM Bicine (pH 9.0)