9X07

Serial Femtosecond Crystallography Structure of Myoglobin from equine skeletal muscle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	PAL-XFEL BEAMLINE NCI
Synchrotron site	PAL-XFEL
Beamline	NCI
Temperature [K]	298
Detector technology	CCD
Collection date	2025-08-20
Detector	RAYONIX MX225-HS
Wavelength(s)	1.3051
Spacegroup name	P 1 21 1
Unit cell lengths	63.690, 28.620, 35.590
Unit cell angles	90.00, 107.19, 90.00

Refinement procedure

Resolution	60.840 - 2.300
R-factor	0.2541
Rwork	0.247
R-free	0.31740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.027
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.720	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	5781	577
<I/σ(I)>	2.07
Completeness [%]	99.2
Redundancy	11
CC(1/2)	0.726	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		298	ammonium sulfate