9WYQ

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 61 2 2
Unit cell lengths	58.690, 58.690, 283.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.345 - 1.700
R-factor	0.2179
Rwork	0.217
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.590	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.121
Number of reflections	33295	2391
<I/σ(I)>	22
Completeness [%]	100.0
Redundancy	36
CC(1/2)	0.999	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.08 M Magnesium acetate tetrahydrate, 0.01 M MES monohydrate pH 6.0, 2.2% Polyethylene glycol 400, 8% w/v Polyethylene glycol 3,350